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Product Introduction:

Pilocarpine Hydrochloride is a selective M3 muscarinic receptor agonist.
Related categories
Signal Pathways>>G Protein Coupled Receptors/G Proteins>>By mAChR
Signal Pathways>>Neural Signal Pathways>>mAChR
Research Fields>>Neurological Diseases
Target point
M3 Muscarinic receiver [1]
In vitro studies were conducted to evaluate the cytotoxicity of pilocarpine by examining the morphology and viability of human corneal stromal (HCS) cells using optical microscopy and MTT assay, respectively. Morphological observations showed that HCS cells exposed to pilocarpine at concentrations ranging from 0.625 to 20g/L exhibited dose-and time-dependent delayed proliferation and morphological abnormalities, such as cell contraction, cytoplasmic vacuolization, detachment from the culture medium, and ultimately death, while no significant differences were observed between pilocarpine at concentrations ranging from 0.625g/L and the control. The MTT assay results showed that after exposure to pilocarpine, the activity of HCS cells decreased to over 0.625 g/L with increasing time and concentration (P<0.01 or 0.05), while the activity of HCS cells treated with pilocarpine was lower than 0.625 g/L, indicating no significant difference in control [2]. Some muscarine agonists, pilocarpine, caused concentration dependent relaxation, with an EC50 of 2.4 mM in the isolated segment of the rat tail artery, which was contracted with deoxepinephrine (10 to 200 nM) [3].
In vivo studies were conducted to detect salivary secretion induced by pilocarpine in control rats (CN) and exercise rats (EX). The saliva volume induced by pilocarpine in EX rats was significantly higher than that in CN rats (P<0.01). On the contrary, the concentration of Na+in the saliva of EX rats was significantly lower than that of CN rats (P<0.05).
Cell viability was determined through MTT assay in cell experiments. In short, HCS cells are treated with 1 × 104 cells/100 μ Inoculate the density of L/well into a 96 well culture plate (Nunc) and culture and treat it. At 4-hour intervals, completely replace the culture medium containing pilocarpine (0.625 to 20g/L) with 100% MTT containing 1.0g/L μ L serum-free DMEM/F12 medium and incubate cells in darkness at 37 ℃. Lasts for 4 hours. After carefully discarding the medium containing MTT, add 150 μ LDMSO was dissolved in darkness at 37 ° C to produce methylcrystals for 15 minutes, and the absorbance at 490nm was measured using a Multiskan GO enzyme-linked immunosorbent assay [2].
Animal experimental rats [1] divided 10-week-old male Wistar rats into one of two groups: exercise (EX, n=6) and control (CN, n=6). Increase EX

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